Volume 40 Issue 3
May  2019
Turn off MathJax
Article Contents
DU Yue, MENG Xiaochen, ZHU Lianqing. Novel algorithm for analysis of cellular fluorescence spectrum based on optimization iteration[J]. Journal of Applied Optics, 2019, 40(3): 461-467. doi: 10.5768/JAO201940.0303001
Citation: DU Yue, MENG Xiaochen, ZHU Lianqing. Novel algorithm for analysis of cellular fluorescence spectrum based on optimization iteration[J]. Journal of Applied Optics, 2019, 40(3): 461-467. doi: 10.5768/JAO201940.0303001

Novel algorithm for analysis of cellular fluorescence spectrum based on optimization iteration

doi: 10.5768/JAO201940.0303001
  • Received Date: 2018-08-13
  • Rev Recd Date: 2019-01-10
  • Publish Date: 2019-05-01
  • When using the spectral measurement method for multi-color fluorescence analysis of cells, the emission spectrum can produce partial spectral overlap, which poses certain difficulties for qualitative and quantitative analysis. Aiming at the problem, a novel algorithm was developed to effectively decompose the overlapped spectral peaks based on optimization iteration. First, the separation model of overlapped peak was built up to obtain the peak vertex of overlapping peaks. And then, according to the size of each constructed peak area, the structure of the constructed peak was re-determined, and finally the vertex and area information of the simulated peak was obtained.Moreover, the algorithm was used to analyze the overlapping peaks formed by the superposition of Gaussian functions, and compared with the conventional methods. The results show that the analytical error of the optimized iterative algorithm is stable within 0.15%, while it can be stabilized within 0.85% after adding random noise, which are all better that the other two algorithms. In addition, the iterative efficiency under the algorithm was calculated, and the results show that the algorithm is 32.2% higher than the conventional method.
  • loading
  • 尚雪梅, 董明利, 潘志康, 等.基于递推最小二乘的荧光补偿参数辨识方法[J].计算机仿真, 2015, 32(5):252-255, 289. doi: 10.3969/j.issn.1006-9348.2015.05.056

    SHANG Xuemei, DONG Mingli, PAN Zhikang, et al. Identification of the parameters of spectral compensation for flow cytometer based on the recursive least squares algorithm[J]. Computer Simulation, 2015, 32(5):252-255, 289. doi: 10.3969/j.issn.1006-9348.2015.05.056
    KAUPPINEN J K, MOFFATT D J, MANTSCH H H, et al. Fourier self-deconvolution:a method for resolving intrinsically overlapped bands[J]. Applied Spectroscopy, 1981, 35(3):271-276. doi: 10.1366/0003702814732634
    黄洪全, 丁卫撑, 龚迪琛, 等.基于统计遗传算法的X射线荧光重叠峰分解[J].光谱学与光谱分析, 2015, 35(8):2320-2323. doi: 10.3964/j.issn.1000-0593(2015)08-2320-04

    HUANG Hongquan, DING Weicheng, GONG Dishen, et al. Decomposition of X-ray fluorescence overlapping peaks based on statistical and genetic algorithms[J]. Spectroscopy and Spectral Analysis, 2015, 35(8):2320-2323. doi: 10.3964/j.issn.1000-0593(2015)08-2320-04
    冯飞, 王府北, 谢非, 等.小波变换与高斯拟合在光谱重叠峰解析中的应用[J].光子学报, 2015, 44(6):0630001-6. http://d.old.wanfangdata.com.cn/Periodical/gzxb201506019

    FENG Fei, WANG Fubei, XIE Fei, et al. Implementation of spectral overlap resolution based on wavelet transforms and gaussian fitting[J]. ACTA PHOTONICA SINICA, 2015, 44(6):0630001-6. http://d.old.wanfangdata.com.cn/Periodical/gzxb201506019
    陶维亮, 刘艳, 王先培, 等.时频域分形维数分析的光谱信号重叠峰解析算法[J], 光谱学与光谱分析, 2017, 37(12):3664-3669. http://d.old.wanfangdata.com.cn/Periodical/gpxygpfx201712003

    TAO Weiliang, LIU Yan, WANG Xianpei, et al. Implementation of overlapping peak separation algorithm for absorption spectra by fractal dimension analysis in time-frequency domain[J], Spectroscopy and Spectral Analysis, 2017, 37(12):3664-3669. http://d.old.wanfangdata.com.cn/Periodical/gpxygpfx201712003
    严彩娟.纵向迭代法及其改进方法分离重叠色谱峰[D].天津: 天津大学, 2008.

    YAN Caijuan. Resolution of overlapping chromatographs by vertically iterative transition and modified vertically iterative transition[D]. Tianjin: Tianjin University, 2008.
    叶国阳, 徐科军.基于色谱重叠峰相似性原理的双重叠峰分峰新方法[J].仪器仪表学报, 2015, 36(2):439-445. http://d.old.wanfangdata.com.cn/Periodical/yqyb201502024

    YE Guoyang, XU Kejun. New decomposition peak methods of overlapping chromatographic peaks based on similarity principle[J].Chinese Journal of Scientific Instrument, 2015, 36(2):439-445. http://d.old.wanfangdata.com.cn/Periodical/yqyb201502024
    朱强, 张荣, 俞建成.基于函数平分迭代的色谱重叠峰分峰方法[J].真空科学与技术学报, 2017, 37(5):544-547. http://d.old.wanfangdata.com.cn/Periodical/zkkx201705018

    ZHU Qiang, ZHANG Rong, YU Jiancheng. Novel algorithm for decomposition of overlapped chromatographic peaks based on function split iteration[J]. Chinese Journal of Vacuum Science and Technology, 2017, 37(5):544-547. http://d.old.wanfangdata.com.cn/Periodical/zkkx201705018
    宋磊, 郑宝忠, 张莹, 等.一种基于高斯混合模型的改进EM算法研究[J].应用光学, 2013, 34(6):985-989. http://www.yygx.net/CN/abstract/abstract10319.shtml

    SONG Lei, ZHENG Baozhong, ZHANG Ying, et al. Advanced EM algorithm based on Gaussian mixture model[J].Journal of Applied Optics, 2013, 34(6):985-989. http://www.yygx.net/CN/abstract/abstract10319.shtml
    林兆培, 李钰, 吴慧文.基于二次微分和小波变换的色谱重叠峰分析[J].华东理工大学学报(自然科学版), 2014, 40(1):91-95. doi: 10.3969/j.issn.1006-3080.2014.01.016

    LIN Zhaopei, LI Yu, WU Huiwen. Analysis of overlapping chromatographic peaks based on quadratic differential and wavelet transform[J]. Journal of East China University of Science and Technology(Natural Science Edition), 2014, 40(1):91-95. doi: 10.3969/j.issn.1006-3080.2014.01.016
    LIU H, PANG Z, FAN G. Translation modification iteration for resolution and quantification of overlapping chromat-ographic peaks[J]. Chromatographia, 2016, 79:1543-1552. doi: 10.1007/s10337-016-3172-3
    王玥, 孟晓辰, 祝连庆.基于脉冲时延估计的荧光寿命表征方法[J].应用光学, 2018, 39(2):207-211. http://www.yygx.net/CN/abstract/abstract11090.shtml

    WANG Yue, MENG Xiaochen.Fluorescence lifetime representation method based on impulse delay estimation[J]. Journal of Applied Optics, 2018, 39(2):207-211. http://www.yygx.net/CN/abstract/abstract11090.shtml
    朱仲良.基于RBF神经网络的重叠峰解析[D].合肥: 安徽大学, 2014.

    ZHU Zhongliang. Overlapping peaks resolve based on RBF neural network[D]. Hefei: Anhui University, 2014.
    李莉莉.基于前期经验的GA重叠峰解析算法研究[D].合肥: 安徽大学, 2018.

    LI Lili. Research on GA overlapped peak analysis algorihm based on previous experience[D]. Hefei: Anhui University, 2018.
    陈锐, 周霖.燃烧产物组成激光诱导荧光光谱的测量[J].应用光学, 2006, 27(5):455-459. doi: 10.3969/j.issn.1002-2082.2006.05.022

    CHEN Rui, ZHOU Lin. Measurement of combustion product composition by laser induced fluorescence spectroscopy[J]. Journal of Applied Optics, 2006, 27(5):455-459. doi: 10.3969/j.issn.1002-2082.2006.05.022
  • 加载中


    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索
    Article views (919) PDF downloads(27) Cited by()
    Proportional views


    DownLoad:  Full-Size Img  PowerPoint