Abstract: With the rapid development of quantum information technology, quantum imaging provides a new approach for achieving high-quality three-dimensional imaging. However, traditional quantum imaging methods are primarily limited to two-dimensional imaging, unable to accurately extract target depth information, and suffer from low reflectivity calculation accuracy. To address this problem, we proposed a reflectivity-assisted three-dimensional quantum imaging method based on entangled photons. By analyzing the distribution of the number of entangled photons during free-space transmission, a photon detection probability model and a coincidence counting probability density function were constructed to estimate the reflectivity of the target surface. Simultaneously, the delay difference matrix was optimized in combination with the coincidence counting value to accurately extract the depth information of the target. The reflectivity was used as the auxiliary information and combined with the depth information to achieve three-dimensional reconstruction of the target. The influence of single-pixel exposure time on the imaging results was simulated and analyzed, and a quantum imaging experimental platform was established to verify the effectiveness and feasibility of the proposed method.